The Luciferase assays are convenient ‘read outs’ for a variety of biological processes. The Gaussia luciferase is a 20kD small protein. It has more advantages over either firefly or Renilla luciferases: 1) higher signal intensity( 1000x ); 2) Secret into the cell culture media, ATP independent, the reporter assay can be monitored live cell in real-time.
We offer Gaussia luciferase reporter plasmid constructions, cloning/synthesis your gene of interesting(GOI) into the pGluci vectors. You just let us know the GOI, we will synthesize the promoter of the GOI for you.
| Cat# | Product | Description | Size | Price |
|---|---|---|---|---|
| L2001 | pGluci reporter | The ready-to-use Gaussia luciferase reporter plasmid with your gene-of-interesting(GOI) insert. This reporter is more than 100-fold sensitive than common luciferases, such as FLuc, RLuci. The reporter is secreted also, can be measured in the medium, blood[ref]. | 10ug lyophilized DNA | $250 (0.25/base) |
| L2002 | pCMV-Gaussia-Flag | The ready-to-use expressing Guassia luciferase plasmid under CMV promoter. It can be a positive control for the assay. | 5ug lyophilized DNA | $49 |
| L2003 | pCMV-β-Gal | The ready-to-use expressing β-galactosidase plasmid. It converts the ONPG substrate to yellow color, as a transfection efficiency control | 5ug lyophilized DNA | $49 |
| L2004 | Lenti-Guassia-reporter | The HIV based self-inactivating lentiviral plasmid. The lenti-Gaussia-reporter is easy to into the suspension cells and stem cells. The Gaussia-reporter will integrate into the genome of the target cells. The cells can be tracking the luciferase activity. | 5ug lyophilized DNA | $250 (0.25/base) |
| L2005 | pCW-tet-On-Gaussia-reporter | The doxycycline-inducible lentiviral Gaussia expression plasmid. | 5ug lyophilized DNA | $250 (0.25/base) |
| L2006 | pCMV-GOI-Gaussia-fusion-Flag | The ready-to-use expressing your gene-of-interesting(GOI) fusion with Gaussia luciferase and Flag-tagged. This plasmid can be used for labeling Influenza A Virus/or SARS-Cov2 proteins to analyze viral replication and Its Inhibition by antiviral Compounds and Cellular Proteins[ref]. | 10ug lyophilized DNA | $250 (0.25/base) |