Two services, built on your own bench experience
Every project is designed and reviewed personally — not run through an automated pipeline.
gRNA design service
Guide RNA designed and validated against your target sequence for cell line engineering, knockouts, or knock-ins.
Custom plasmid builds
Subcloning, tag-protein fusion, and CAR-T plasmid construction — built to your construct map, with sequence confirmation before delivery.
Cas9 and Cpf1, in the format your protocol needs
Protein, linearized RNA, or circRNA — pick the format that fits your delivery method.
Cas9 protein
Purified Cas9 protein for RNP delivery — pairs with your gRNA for electroporation or microinjection workflows.
Ask about thisCas9 linearized RNA
Linearized mRNA for Cas9 expression, ready for in vitro transcription or direct transfection.
Ask about thisCas9 circRNA
Circular RNA format for Cas9 expression — improved stability over linear mRNA for extended expression windows.
Ask about thisCpf1 protein
Purified Cpf1 (Cas12a) protein for RNP delivery, for AT-rich targets where Cas9 PAM constraints don't fit.
Ask about thisCpf1 linearized RNA
Linearized mRNA for Cpf1 expression, ready for in vitro transcription or direct transfection.
Ask about thisCpf1 circRNA
Circular RNA format for Cpf1 expression — improved stability over linear mRNA for extended expression windows.
Ask about thisSequence submitted
Tell us the target and the edit you need — no fixed order forms required.
Design and synthesis
Constructs are designed and built in-house, with QC before anything ships.
Delivered to your bench
Ready-to-use format, with the documentation your protocol needs.
Send us your sequence, get a quote back fast.
Most requests get a response within one business day — plasmid or ready-to-use, your call.
Contact the teamTwo services, built on your own bench experience
Every project is designed and reviewed personally — not run through an automated pipeline.
gRNA design service
Guide RNA designed and validated against your target sequence for cell line engineering, knockouts, or knock-ins.
Custom plasmid builds
Subcloning, tag-protein fusion, and CAR-T plasmid construction — built to your construct map, with sequence confirmation before delivery.
Cas9 and Cpf1, in the format your protocol needs
Protein, linearized RNA, or circRNA — pick the format that fits your delivery method.
Cas9 protein
Purified Cas9 protein for RNP delivery — pairs with your gRNA for electroporation or microinjection workflows.
Ask about thisCas9 linearized RNA
Linearized mRNA for Cas9 expression, ready for in vitro transcription or direct transfection.
Ask about thisCas9 circRNA
Circular RNA format for Cas9 expression — improved stability over linear mRNA for extended expression windows.
Ask about thisCpf1 protein
Purified Cpf1 (Cas12a) protein for RNP delivery, for AT-rich targets where Cas9 PAM constraints don't fit.
Ask about thisCpf1 linearized RNA
Linearized mRNA for Cpf1 expression, ready for in vitro transcription or direct transfection.
Ask about thisCpf1 circRNA
Circular RNA format for Cpf1 expression — improved stability over linear mRNA for extended expression windows.
Ask about thisSequence submitted
Tell us the target and the edit you need — no fixed order forms required.
Design and synthesis
Constructs are designed and built in-house, with QC before anything ships.
Delivered to your bench
Ready-to-use format, with the documentation your protocol needs.
Send us your sequence, get a quote back fast.
Most requests get a response within one business day — plasmid or ready-to-use, your call.
Contact the team