| Partner/tag | MW(kD) | Pros | Cons | Purification |
| Strep-Tactin | <1 | The smal Strep-tagII (WSHPQFEK. 8aa) can be used to purify active, native conformation proteins for high purity on one-step Strep-Tactin bead from mammalian and bacteria. It can be combined with Hisx6-tag for a standardized two-step purification. It is most useful for active enzyme purification; IP for MS protein-protein interaction. | Strep-Tactin beads are a little more expensive. | Strep-tactin( engineered strepadvidin) bead, You can check IBA, Giagen, or GE life science for more detail |
| Estrogen receptor(ERT2) | 35 | The ERT2 is a mutant form of the human estrogen receptor which does not bind its natural ligand (17β-estradiol), but binds the synthetic estrogen receptor ligands 4-hydroxytamoxifen (OHT or tamoxifen). The ERT2-fusion protein can translocate to the nucleus by tamoxifen administration. | | |
| CD25 | | The CD25 human is a surface receptor on activated T cell and most B-cell neoplasms. The extracellular regions and transmembrane regions of CD25 alpha can be fused with intracellular signalling molecules for receptor signalling studies. The CD25-Fc fusion protein has potent antitumor activity(see Nature) | | |
| Anti-CD19 | 29 | Anti-CD19 scFv is an attractive reagents for treating CD19-positive lymphoma and autoimmune diseases. The anti-CD19 chimeric antigen receptor T- cell(CAR-T) therapy is being expected for B-cell leukemia and lymphoma treatment. The CAR(chimeric antigen receptor) consists of anti-CD19 scFv that recognizes antigen, an extracellular hinge linker, a transmembrane domain and an intracellular signaling domain such as CD3zeta, CD28 and 4-1BB. The P&A biotech provides anti-CD19 chimeric antigen receptor (CAR) construct service. Please contact us for more information. | | |
| VP16-AD(413-490) | 8 | VP16 is is a trans-acting protein from herpes simplex virus. The VP16-AD-fusion protein can recruit the active complex for gene transcription. | | |
| BirA | 35 | The BirA is an E. coli biotin ligase, and specifically, conjugate biotin to the 15 amino acids AviTag peptide(GLNDIFEAQKIEWHED). | | |
| TAT | <1 | TAT(trans-acting activator of transcription) is 10 aminino-acid peptide from HIV-1. The Tat-fusion protein allows for the rapid introduction of the fusion proteins into primary and transformed cells and locates into nucleus aslo. Methoths | | |
| Gal4-DBD(1-147) | 16 | In Gal4-UAS system, Gal4-DBD-fusion protein, binds to a specific 17bp nucleotide sequence. the effect of the fusion protein on transcription activation/repression can be measured by reporter(e.g. luciferase, GFP) for functional domains, protein-protein interactions, and site-specific proteolysis. | | |
| Fc(h IgG Ch2ch3) | 25 | Human Fc (IgG1 Fc-Ch2-Ch3 regions) can strongly bind to Fc receptors on B cell, NK cell and macrophages and to protein A/G. These valuable features enable Fc fusion proteins for prolong the half-life and an improved therapeutic efficacy. | | Fc-fusion protein can be easyly purifed by protein A bead. The receptor-Fc fusion can be in a dimeric form, which activates and enhances its affinity for the cognate ligand. |
| Cre recombinase | 35 | Cre Recombinase is an enzyme from bacteriophage P1 that catalyzes the LoxP sites. The Cre has been widely used to generate a tissue-specific or inducible knockout in transgenic mice or conditional gene expression in cells | | |
| HA | <1 | | | Anti-HA |
| Hisx6 | <1 | | | |
| CBP | 4 | | Purity differs greatly | Calmodulin bead |
| Trx | 12 | | Does not work well with larger MW target proteins | N/A |
| DsbA | 23 | | Periplasmic | N/A |
| NusA | 55 | | | |
| MBP | 43 | | large tag | Amylose bead |
| GST | 26 | | | Glutathione bead |
| TEV | <1 | | | need affinity tag |
| SUMO | 12 | | | need affinity tag |
| Flag | 1 | | Expensive and elution can be a problem occasionally | Anti- Flag-M2 bead |
| eGFP | 27 | | | |
| eYFP | 27 | | | |
| eCYP | | | | |
| eBFP | 27 | | | |
| RFP | | | | |
| mCherry | | | | |
| DsRed | | | | |
