Ready-for-use mRNA for protein expression and vaccination; gRNA for cell line or zygote micro-injection.
1. mRNA vector for protein expression and vaccination.
Please request a quote here.
The synthetic mRNA has been used for human iPS generating (Nature protocols 1969 citations), and also for viral vaccination (see the new publications for mRNA vaccination in Cell and Nature).
We provide:
1)Synthesis and optimization of cDNA template for expression protein service, $0.25/bp.
2)Synthetic mRNA production, $199/20μg.
3)Document with the sequence in the plasmid vector. Synthetic mRNA products are ready for protein expression, virus vaccination (CNN report-1) and cancer vaccination (CNN report-2).
See the synthetic mRNA products for iPSC here.
2. T7-gRNA plasmid with T7-promoter in front of your specific gRNA ($99/each, Cat #R16003).
This plasmid is ready for in vitro transcription. We design and confirm the sequence with you. You can prepare your gRNA via T7 RNA polymerase transcription kit, or purchase from our services. For in vitro gRNA target digestion, please click here.
Delivery: 1) pUC-T7-gRNA plasmid, 100ng in TE buffer, 2) report with sequence validation and related genomic sequence.
3. gRNA synthesis (105nt CrRNA-TracrRNA for Cas9, 42nt CrRNA for Cpf1) Price: $199/each, Cat#R16004
This is a ready-to-use gRNA. You just simply mix this specific gRNA with our Cas9 nuclease/ or nickase, Cpf1 in vitro and micro-inject into the ES cells or zygote. For vitro gRNA target sequence digestion and microinjection information, please click here.
Delivery: 1. pUC-T7-gRNA plasmid and sequence validation, 2. Your gRNA polynucleotides [ 105nt CrRNA-TracrRNA, or 42nt CrRNA], 50μg in TE buffer, or lyophilized powder.
(4) All-in-one Crispr-Cas9-gRNA plasmid ($99/each, Cat#D17001)
This is an all-in-one plasmid containing your gRNA and cas9 with puromycin selection. This plasmid is easy for setting up a cell lines.
Delivery: 1) ready to use all-in-one Crispr-Cas9-gRNA plasmid, 100ng in TE buffer, 2) report with sequence validation and genomic sequence.
All the gRNAs that we design for your genes will be confirmed with you. Please ask for a quote; a complementary discount will be provided for first order.