Crispr-Cpf1 - P&A Biotech

Our Price	$199/$299
Quantity	50 μg/100 μg(2mg/ml)
Description	Cpf1 is an RNA-guided nuclease, similar to cas9. Cpf1 requires only one RNA(42nt) and cleaves DNA in a staggered pattern. Two Cp1-family proteins, AsCpf1 and LbCpf1, have been shown to perform efficient genome editing in human cells. Both AsCpf1 and LbCpf1 ribonucleoproteins (RNPs) had no off-target editing in a human cell culture model (ref1). Cpf1 enzyme cuts both RNA and DNA(Nature)
Application	Used for 1) In vitro DNA and RNA cleavage by gRNA guiding; 2) Genome editing through the injection for mammalian zygote, zebrafish embryos, & drosophila embryos; 3) Introduction into cell by transfection. In this buffer, AsCpf1 can directly be used for microinjection, eletroporation and in vitro DNA cleavage.
Source	Humanized AsCpf1 gene from an E. Coli K-12 strain
Physical Appearance	In the buffer (20 mM Hepes pH7.5, 200 mM NaCl, 1 mM MgCl2, 5% glyerol, 1mM TCEP). 0.2μm filter to sterilize.
Formulation	The protein concentrations can be at 2mg/ml the buffer.
Solubility	The AsCpf1 is very soluble in water and most aqueous buffers.
Stability	Although stable at room temperature, AsCpf1 should be stored desiccated below 0°C.
Purity	Greater than 98.0% as determined by SDS-PAGE.
Amino acid sequence	The N terminus contains a 6xHis-tag and the C-terminus contains an SV40NLS peptide for the nuclear localization signal.
Biological Activity	Biological activity is assayed by in vitro gRNA guide DNA cleavage.
Protein content	Protein purity of 98%. Quantification was carried out by Bradford methods.
References	1. Cpf1 update 2. Genome-wide analysis reveals specificities of Cpf1 endonucleases in human cells(Kim lab) 3. Genome-wide specificities of CRISPR-Cas Cpf1 nucleases in human cells( Joung lab)
Usage	P&A Biotech’s products are furnished for laboratory research use only.