CRISPR/Cas9 and mRNA products

Cat #ProductsDescriptionSizePrice
P15001Cas9 protein in buffer[20 mM Hepes pH 7.5, 150 mM KCl, 1 mM MgCl2, 5%glycerol, 1 mM Tris(2-chloroethyl) phosphate (TCEP)].Contains nuclear localization signal(NLS) peptides and 6xHis-tag. 0.2µm filter to sterlize. 50µg(1mg/mlx50µl)
100µg(2mg/mlx50µl) 		$199
$299
P15002Cas9D10A protein in buffer[20 mM Hepes pH 7.5, 150 mM KCl, 1 mM MgCl2, 5%glycerol, 1 mM Tris(2-chloroethyl) phosphate (TCEP)].Contains nuclear localization signal(NLS) peptides and 6xHis-tag. 0.2µm filter to sterlize. 50µg(1mg/mlx50µl)$199
P15003Cas9 protein in buffer[20 mM Hepes pH 7.5, 150 mM KCl, 1 mM MgCl2, 10%glycerol, 1 mM Tris(2-chloroethyl) phosphate (TCEP)].Contains nuclear localization signal(NLS) peptides and 6xHis-tag. 0.2µm filter to sterlize.100 µg(5mg/mlx20µl)$299
P15004Cas9D10A protein in buffer[20 mM Hepes pH 7.5, 150 mM KCl, 1 mM MgCl2, 10%glycerol, 1 mM Tris(2-chloroethyl) phosphate (TCEP)].Contains nuclear localization signal(NLS) peptides and a 6xHis-tag. 0.2µm filter to sterlize.100 µg(5mg/mlx20µl)$299
P15005Cas9 protein in transduction buffer(25 mM NaH2PO4,pH8.0, 500 mM NaCl, 250 mM NDSB-201, 150 mM glycerol, 75 mM glycine, 1.25 mM MgCl2, 1 mM 2-mercaptoethanol).Contains nuclear localization signal(NLS) peptides and 6xHis-tag in transduction buffer. 0.2µm filter to sterlize. See Cell for details.250µg(5mg/mlx50µl)$699
P15006Cas9D10A protein in transduction buffer(25 mM NaH2PO4 pH8.0, 500 mM NaCl, 250 mM NDSB-201, 150 mM glycerol, 75 mM glycine, 1.25 mM MgCl2, 1 mM 2-mercaptoethanol).Contains nuclear localization signal(NLS) peptides and 6xHis-tag in transduction buffer. 0.2µm filter to sterlize.250µg(5mg/mlx50µl)$699
P15007dCas9(D10A/H840A) protein in buffer[20 mM Hepes pH 7.5, 150 mM KCl, 1 mM MgCl2, 10%glycerol, 1 mM Tris(2-chloroethyl) phosphate (TCEP)].Contains nuclear localization signal(NLS) peptides and a 6xHis-tag. 0.2µm filter to sterlize.50µg(1mg/mlx50µl)$199
P15008dCas9(D10A/N863A) protein in transduction buffer(25 mM NaH2PO4 pH8.0, 500 mM NaCl, 250 mM NDSB-201, 150 mM glycerol, 75 mM glycine, 1.25 mM MgCl2, 1 mM 2-mercaptoethanol).The dCas9 is a deactivated enzyme, which contains nuclear localization signal(NSL) peptides and 6xHis-tag. 0.2µm filter to sterlize.250µg(5mg/mlx50µl)$699
P15009SaCas9 protein in buffer[20 mM Hepes pH 7.5, 150 mM KCl, 1 mM MgCl2, 1 mM Tris(2-chloroethyl) phosphate (TCEP)].SaCas9 enzyme from Staphylococcus aureus, smaller than the common SpCas9 from Staphylococcus pyogenes. The SaCas9 recognizes the NGRRT/NNGRR PAM sequence and is effectively delivered. See Nature or The Scientist for details. It contains nuclear localization signal(NSL) peptides and a His-tag in transduction buffer. 0.2µm filter to sterlize.50µg(1mg/mlx50µl)$199
P15010AsCpf1 protein in buffer[20 mM Hepes pH 7.5, 150 mM KCl, 1 mM MgCl2, 5%glycerol, 1 mM Tris(2-chloroethyl) phosphate (TCEP)].Cpf1, a new nuclease protein for genome editing , Contains nuclear localization signal(NLS) peptides and a 6xHis-tag. 0.2µm filter to sterlize.50µg(2mg/mlx25µl)
100µg(2mg/mlx50µl) 		$199
$299
R16001Cas9 mRNAThe Cas9 mRNA is capped and poly-A tailed. Ready for injection or transfection.30µg in lyophilized powder or TE buffer$199
R16002Cas9 D10A mRNAThe Cas9 D10A mRNA is capped and poly-A tailed. Ready for injection or transfection.30µg in lyophilized powder or TE buffer$199
R16003T7-gRNA plasmidWe design( or you can provide the sequence for us) and clone your specific gRNA in the ready to use vector and with the sequence confirmation.100ng in TE buffer$99/each, more than two plasmids
R16004gRNA oligonucleotidesThe customized gRNA oligonucleoptides ( 105nt crRNA-tracrRNA for Crispr-Cas9; 42nt crRNA for Crispr-Cpf1) with sequence confirmation, Ready for injection or transfection.50µg in lyophilized powder or TE buffer$199
R16005Custom Synthetic mRNA or long non-coding RNA service. This mRNA with cap, 5'-UTR, 3'-UTR and poly-A, and ready for protein expression.The service including: 1) optimizing the cDNA coding and cloning into pUC vector with T7-promoter; 2) synthetic mRNA; 3) your mRNA with T2A peptide eGFP for tracking expression. See detail here 1) 100ng pUC-cDNA in TE buufer; 2) 50µg synthtic mRNA or LnRNA in lyophylized powder1)$0.25/bp DNA, more than 1kb; 2) $199/ 50µg mRNA
D17001Crispr-Cas9-gRNA plasmidThe all-in-one crispr-Cas9-gRNA plasmid contains U6 promoter and puromycin selection. The plasmid is easy for gene knock-out or knock-in cell line setup. We can provide technical support for cell line setup. 100ng in TE buffer$99/each, more than two plasmids
D17002Donor DNA knock-in fragmentThe donor DNA fragment (500-1kb) in pUC19 vector. It contains restriction site for knock-in screen using PCR product digestion. 100ng in TE buffer$199/each, more than two plasmids
D17003T7-DNA fragment for gRNATheDNA fragment are temperates for cloning-free sgRNA synthesis or high-throughput mutagenesis. The DNA fragment contains T7 promter for in vitro transcription and your specific gRNA sequence.100ng in TE buffer$79/each, more than three